Ex Vivo Culture of Carotid Atherosclerotic Plaques

Lasse Lorentzen

Proteomics of carotid plaques

Proteomics of carotid plaques

Proteomics of carotid plaques

Degradomics of carotid plaques

Degradomics of carotid plaques

Degradomics of carotid plaques

Translational research

Animal models in translational research

Ex vivo cultured carotid plaques

An alternative to animal models

Ex vivo cultured carotid plaques

Combination with proteomics

Ex vivo cultured carotid plaques

Potenital uses:

  • Basic research
    • What proteins are actually expressed in carotid plaques
    • Effect of oxygen level
    • Effect of growth factors and cytokines
  • Test new compounds (e.g. pipeline drugs)
  • Mechanistic studies of approved drugs
  • Knock-out of proteins of interest with PROTACS

Ex vivo cultured carotid plaques

Potenital uses:

  • Basic research
    • What proteins are actually expressed in carotid plaques
    • Effect of oxygen level
    • Effect of growth factors and cytokines
  • Test new compounds (e.g. pipeline drugs)
  • Mechanistic studies of approved drugs
  • Knock-out of proteins of interest with PROTACS

Results

Incorporation of heavy-labelled amino acids

Incorporation of heavy-labelled amino acids

Most/least newly synthesized molecular functions

Synthesis of apolipoproteins

Differential synthesis in a mixed plaque

Proteins synthesized more in hard pieces

Differential synthesis in a mixed plaque

Proteins synthesized more in hard pieces

Ex vivo cultured carotid plaques

Potenital uses:

  • Basic research
    • What proteins are actually expressed in carotid plaques
    • Effect of oxygen level
    • Effect of growth factors and cytokines
  • Test new compounds (e.g. pipeline drugs)
  • Mechanistic studies of approved drugs
  • Knock-out of proteins of interest with PROTACS

Ex vivo cultured carotid plaques

Potenital uses:

  • Basic research
    • What proteins are actually expressed in carotid plaques
    • Effect of oxygen level
    • Effect of growth factors and cytokines
  • Test new compounds (e.g. pipeline drugs)
  • Mechanistic studies of approved drugs
  • Knock-out of proteins of interest with PROTACS

Ex vivo cultured carotid plaques

Potenital uses:

  • Basic research
    • What proteins are actually expressed in carotid plaques
    • Effect of oxygen level
    • Effect of growth factors and cytokines
  • Test new compounds (e.g. pipeline drugs)
  • Mechanistic studies of approved drugs
  • Knock-out of proteins of interest with PROTACS

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